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A Tubulin Binding Peptide Targets Glioma Cells Disrupting Their Microtubules, Blocking Migration, and Inducing ApoptosisRaphael Berges, Julien Balzeau, Alan C Peterson and Joel EyerEffects of the NFL TBS.40 63 peptide on the microtubule cytoskeleton of rat glioma cells and primary astrocytes and neurons. Rat Authenitc Authenitc Red Miikka Kiprusoff Jersey Red Devin Setoguchi Jersey F98 glioma Elite White Devin Setoguchi Jersey cells and rat primary astrocytes or neurons were grown in the presence of NFL TBS.40 63 or NFL SCR peptides (10 for 6 hours. Microtubules were detected Elite Johnny Gaudreau Jersey Men Jarome Iginla Jersey using an antitubulin antibody (red), and the biotinylated NFL TBS.40 63 Authenitc TJ Brodie Jersey peptide was detected using Alexa labeled avidin (green). Elite Devin Setoguchi Jersey Cells were examined with a Authenitc Mason Raymond Jersey confocal microscope and numerous fluorescent aggregates, corresponding Authenitc Red Jarome Iginla Jersey to Elite Red Kris Russell Jersey the biotinylated peptide typically were observed. Glioma cells containing NFL TBS.40 63 lack a normal microtubule network and typically assume a spherical Elite White TJ Brodie Jersey shape. Except for rare cells demonstrating one or two relatively weakly labeled aggregates, primary rat astrocytes or neurons cultured under similar conditions remained unlabelled and their microtubule networks appeared unaltered. Incubation Authenitc White Brandon Bollig Jersey with the scrambled NFL SCR peptide did not alter microtubule organization in astrocytes, neurons or glioma cells. White bars, 10 NFL, neurofilament light; SCR, scrambled; TBS, tubulin binding site.
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